![]() The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All reported data have been deposited in the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) under BioProject accession number PRJNA453789.įunding: This work was supported by the National Institute of Health U42 OD010918 to CLF. Received: Accepted: OctoPublished: October 25, 2018Ĭopyright: © 2018 Bidot et al. Wilson, University of Illinois at Urbana-Champaign, UNITED STATES Subsequent PERMANOVA and PCoA of cecal data revealed that several combinations of bedding and water decontamination methods resulted in differing GM, highlighting the complexity by which environmental factors interact to modulate GM.Ĭitation: Bidot WA, Ericsson AC, Franklin CL (2018) Effects of water decontamination methods and bedding material on the gut microbiota. Two factor PERMANOVA of cecal GM data revealed significant changes when comparing bedding and water decontamination methods, while no significant effects were noted in the fecal GM data. Sequence data were analyzed using Qiime and groups were compared using principal coordinate analysis (PCoA) and permutational multivariate analysis of variance (PERMANOVA). ![]() DNA was extracted from samples, amplified by PCR using conserved bacterial primer sets and subjected to next generation sequencing. Feces was collected at day 0, and at day 28 (endpoint), fecal and cecal samples were collected. To this end, Crl:CD1 (ICR) mice were housed on corn cob or compressed paper chip bedding and provided water that was decontaminated by four different methods: autoclaving with reverse osmosis, autoclaving with hydrochloric acid, autoclaving with sulfuric acid, and autoclaving alone. The objective of this study was to expand these studies to examine the effect of additional bedding materials and methods of water decontamination on GM diversity and composition. We and others have also shown that a number of factors associated with rodent research, including vendor, cage system, and bedding can alter GM. Our laboratory has shown that differences in gut microbiota (GM) can modulate phenotypes of models of colon cancer and inflammatory bowel disease. Unfortunately, many models suffer from lack of phenotype reproducibility. Rodent models are invaluable to understanding health and disease in many areas of biomedical research.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |